Fluorescent Cationic Fluorinated Oxazoliniums for Cysteine Bioconjugation via S N Ar Reaction

Key Points

• To develop cationic fluorinated oxazolinium compounds for specific bioconjugation to cysteine residues.
• Synthesize cationic fluorinated oxazolinium compounds.
• Conduct SNAr reaction under mild conditions.
• Test bioconjugation efficiency on peptides and proteins.
• Achieved fluorescent labeling with conversion rates up to 99%.
• Demonstrated successful bioconjugation in live cell environments.
• Showed moderate to excellent efficiency of the fluorescent compounds.