Functional microarray biochips promote micropatterned adhesion-cytoskeleton-nuclear coupling to guide endothelial force-sensing mechanotransduction

Key Points

• The aim is to explore how microarray biochips influence endothelial cell behaviors through spatial control.
• Utilized microarray biochips to create controlled microenvironments for endothelial cells.
• Examined cell adhesion, morphology, and mechanosensitive signaling under varied geometric constraints.
• Analyzed the effects of geometric variations on cytoskeleton-Nuclear coupling.
• Enhanced cell adhesion was observed on micropatterned surfaces compared to non-patterned.
• Significant changes in cytoskeleton organization were linked to shapes defined by biochips.
• Mechanotransduction signaling pathways were activated differently based on geometric constraints, indicating guided force-sensing.