Development of in situ refolding technology for directed evolution of enzymes from eukaryotes

Key Points

• Directed evolution enhances enzyme performance, leading to better catalytic efficiency.
• Key evidence includes improved activity metrics across various enzyme classes, demonstrating substantial gains.
• Assessment using directed evolution and in situ refolding explores enzyme adaptability in eukaryotic systems.
• May enable efficient enzyme design for industrial applications with potential biotechnological impact.