This article describes in detail the elements of a magnetic deep eutectic solvent-based vortex-assisted liquid liquid microextraction (MDES-VA-LLME) procedure for the extraction and quantification of 4-methylimidazole (4-MeI) from food and beverage matrices. Phase separation was achieved with a strong magnet. The MDES-VA-LLME procedure does not include additional experimental steps such as heating or centrifugation to ensure phase separation. Therefore, the procedure enables the rapid and low-cost extraction of 4-MeI. Optimization step was carried out using the Box–Behnken design. The MDES-VA-LLME was linear between 25 and 900 µg L −1 (R 2 = 0.9921). Relative standard deviations of the presented procedure were acceptable for intraday (1.2–1.8%, N= 5) and interday (1.6–2.3%, N=5×3) studies, with a low detection limit (7.4 µg L −1 ) and satisfactory relative recovery (92.5-98.1%). The accuracy of the MDES-VA-LLME procedure was compared with the reference method. In the final stage, the MDES-VA-LLME procedure was successfully applied to different in food and beverage matrices. • Magnetic DES-based vortex microextraction was developed for 4-methylimidazole. • The magnetic DES holding 4-methylimidazole was separated using a strong magnet. • Optimization step was carried out using the Box–Behnken design. • The method is green (AGREE score: 0.71), fast, and needs no centrifugation or heating. • The method showed high enrichment factor (133) and low LOD (7.4 µg/L).
Fesliyan et al. (Wed,) studied this question.