Sequential digestions enable identification and quantification of rapid aspartic acid isomerization in the CDR of a monoclonal antibody light chain | Synapse
March 3, 2026
Sequential digestions enable identification and quantification of rapid aspartic acid isomerization in the CDR of a monoclonal antibody light chain
Key Points
Rapid isomerization of aspartic acid occurs in the complementarity-determining region (CDR) of monoclonal antibodies.
Isomerization affects antibody stability and function, indicating a significant structural impact.
Sequential digestions were employed to accurately quantify the levels of isomerization in the light chain.
Findings suggest that monitoring isomerization may be crucial for improving antibody development and treatment efficacy.