DPPH Rf-shift assay to overcome spectral interference: A rapid, portable TLC-based approach for semi-quantitative antioxidant evaluation

Key Points

• Antioxidant evaluation is enhanced with a rapid TLC-based DPPH Rf-shift assay, addressing spectral interference.
• The DPPH Rf-shift method exhibits a semi-quantitative capacity, obtaining reliable antioxidant measures in varied conditions.
• Portable design supports field deployment of the TLC method, enabling instant antioxidant assessments on-site.
• Findings indicate a promising alternative for antioxidant studies, potentially expanding application in various sectors.