Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy

Key Points

• Molecular analysis focuses on mRNA levels during endoreplication phases in Arabidopsis thaliana.
• The protocol includes a sorting strategy for precise nuclei extraction and analysis of gene expression.
• Employing a systematic approach, the extraction of nuclei facilitates better observation of endoreplication dynamics.
• This protocol may help improve understanding of endoreplication mechanisms and their implications for plant biology.