Integrated computational and experimental identification of high-affinity aptamers for aflatoxin B1 detection

Key Points

• High-affinity aptamers were successfully identified, enabling improved aflatoxin B1 detection methods.
• A critical finding was the top-performing aptamer exhibiting a binding affinity of 0.1 nM, providing robust detection.
• Integration of computational methods with experimental validation facilitated the aptamer selection process effectively.
• This approach highlights the potential of using aptamers in food safety and health monitoring applications.