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March 3, 2026
A one-step, extraction-free RPA-CRISPR/EsCas13d platform for rapid, sensitive and visual detection of PCV2
JD
Jia-qi Duan
Sichuan Agricultural University
YD
Yang-ming Dai
Sichuan Agricultural University
TX
Tong Xu
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Key Points
Rapid detection of PCV2 achieved using a one-step RPA-CRISPR/EsCas13d platform—offering high sensitivity.
Sensitivity of the method allows for effective detection, crucial for timely responses in veterinary diagnostics.
Implementation of the extraction-free approach streamlines the process, reducing time and resource requirements.
The technique may enhance diagnostics in veterinary medicine, particularly for PCV2-related diseases.
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Duan et al. (Fri,) studied this question.
synapsesocial.com/papers/69a75f74c6e9836116a2ad8b
https://doi.org/https://doi.org/10.1016/j.microc.2026.116967
A one-step, extraction-free RPA-CRISPR/EsCas13d platform for rapid, sensitive and visual detection of PCV2 | Synapse