A class-specific and uniform dihydrofolate reductase-mediated fluorescence polarization immunoassay for the detection of antibacterial synergists

Key Points

• The immunoassay detects antibacterial synergists effectively with a specific focus on dihydrofolate reductase.
• Sensitivity was validated through varied instances with a strong correlation observed.
• Employing fluorescence polarization, the method highlights the accurate identification of class-specific responses.
• This work supports a new diagnostic tool for identifying bacterial resistance, enhancing treatment strategies.