circ_0041150 inhibits pancreatic adenocarcinoma cell proliferation and triglyceride accumulation by regulating the miR-1178-3p/AADAC axis.
Does circ_0041150 regulate cell proliferation and triglyceride accumulation in pancreatic adenocarcinoma cells?
circ_0041150 inhibits pancreatic adenocarcinoma progression and triglyceride accumulation by regulating the miR-1178-3p/AADAC axis, offering potential diagnostic and therapeutic targets.
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Triglyceride (TG) metabolism and circular RNAs (circRNAs) are implicated in pancreatic adenocarcinoma (PAAD), but their interconnections remain unclear. This study aims to elucidate the role of hsacirc₀041150 (circ₀041150) in metabolic mechanism and PAAD progression. RNA/protein levels were detected by qRT-PCR/Western blot. Cell proliferation was evaluated using CCK-8 and EdU assays. TG accumulation was assessed via BODIPY 493/503 staining and a commercial TG assay kit. Interactions among circ₀041150, miR-1178-3p, and AADAC mRNA were analyzed via bioinformatics tools, RNA pull-down, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays. circ₀041150 was downregulated in PAAD tissues and cell lines, and its expression correlated with key clinicopathological features of PAAD. Mechanistically, circ₀041150 acts as a competing endogenous RNA (ceRNA) to sponge miR-1178-3p, thereby upregulating AADAC expression, and inhibiting TG accumulation and PAAD cell proliferation. Knockdown of circ₀041150 promoted proliferation and TG accumulation, and these effects were partially reversed by miR-1178-3p inhibition or AADAC overexpression. circ₀041150 regulates AADAC by sponging miR-1178-3p, inhibiting PAAD progression and TG accumulation, and providing diagnostic and therapeutic targets via the circ₀041150/miR-1178-3p/AADAC axis.
Zhao et al. (Thu,) reported a other. circ_0041150 inhibits pancreatic adenocarcinoma cell proliferation and triglyceride accumulation by regulating the miR-1178-3p/AADAC axis.