Modeling retention time distribution in continuous Protein A affinity capture for materials traceability and diversion: A case study with twin-column system

Key Points

• Retention time distribution is critical for ensuring accurate protein capture in bioprocesses.
• The study identifies key factors influencing the efficiency of affinity capture using a twin-column system.
• Modeling approaches highlight potential improvements for materials traceability and minimizing diversion risk.
• Enhanced understanding may enable more effective methods in protein purification and biomanufacturing processes.