Shrew is an essential, recently evolved, Drosophila specific gene required for formation of the embryonic BMP activity gradient

Patterning the dorsal surface of the Drosophila blastoderm embryo requires the rapid redistribution of a BMP heterodimer (Decapentaplegic/Screw) from lateral regions of the embryo to the dorsal midline as cellularization is completed. BMP redistribution creates a steeply graded signal with peak activity in the mid-dorsal region and requires four additional secreted gene products: Short gastrulation (Sog), Twisted gastrulation (Tsg), Tolloid (Tld), and Shrew (Srw). While the functions of Sog, Tsg, Tld, and their vertebrate homologs, in generating BMP activity gradients have been well-described, the role of Drosophila Srw remains an enigma. Here we show that Srw encodes a secreted, N-terminally truncated paralog of Tsg that, like Tsg, stimulates the cleavage of Sog by Tld. However, unlike Tsg, it does not form a stable interaction with any identified component of the patterning machinery. Structural modeling and mutant analysis suggest Srw transiently binds to the Sog/Tsg/BMP complex to facilitate proteolysis of Sog. We further demonstrate that the requirement for Srw can be bypassed by providing additional Tsg in srw mutant embryos prior to cellularization. Phylogenetic analysis suggests that Tsg and Srw emerged from separate duplications of the Crossveinless (Cv) paralog in higher Diptera, and then Drosopholid lineages respectively, thus providing components for progressive specialization of insect embryo patterning. Taking into consideration our genetic, biochemical and phylogenetic data, we present three possible mechanistic models for how Srw might accelerate Sog cleavage by Tld, to hasten BMP gradient formation in rapidly developing Drosophila embryos. • Shrew is required for proper BMP gradient formation in the early Drosophila embryo • Shrew encodes a secreted, N-terminally truncated, Twisted Gastrulation-like protein • Shrew is not a stable component of the Sog-Tsg-BMP complex • Shrew can stimulate processing of the Sog-Tsg-BMP complex by Tolloid • Excess Tsg mRNA rescues shrew mutants to viability