ABSTRACT Progestins are a representative steroid sex hormone widely used in modern contraception and hormone replacement therapy. The accurate monitoring of its concentration in both biological and environmental matrices is crucial for safeguarding reproductive health, optimizing therapeutic regimens, and assessing environmental pollution. In this study, a magnetic covalent organic polymer was synthesized using a simple one‐step method and employed as an adsorbent for magnetic solid‐phase extraction to efficiently extract progestins from biological and environmental samples. This material was synthesized in just 3 h and provided abundant active sites, which enabled high extraction efficiency and yielded enrichment factors of 37–44 in only 10 min. Furthermore, the material exhibited excellent stability and reusability. The adsorption behaviors of the three target drugs on the material were well‐described by the Freundlich isotherm and pseudo‐second‐order kinetic models. Coupling magnetic solid‐phase extraction with high‐performance liquid chromatography achieved excellent analytical performance, including good linearity ( R 2 > 0.9998), low detection limits (0.15–0.18 ng/mL), and high precision (relative standard deviation ≤5.22%). This method was successfully applied to the rapid determination of progestins in lake water and plasma samples, yielding recoveries of 96.1%–102.6% and 99.0%–103.6%, respectively. In conclusion, the proposed method is highly sensitive, reproducible, and practical, showing great potential for the preconcentration and determination of trace progestins in complex samples.
Huang et al. (Sun,) studied this question.