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This study investigates how electroacupuncture affects colitis and the role of vagal cholinergic pathways in mice.

March 14, 2026Journal of Inflammation Research1 citationsOpen Access

Electroacupuncture Attenuates Colitis in Mice Through Activation of Vagus Cholinergic Antiinflammatory Pathways

ZQZhengyang QuChinese Academy of Medical Sciences & Peking Union Medical College MLMin LuoChinese Academy of Medical Sciences & Peking Union Medical College XLXiaoyu LiuChinese Academy of Medical Sciences & Peking Union Medical College

Key Points

This study investigates how electroacupuncture affects colitis and the role of vagal cholinergic pathways in mice.
Mice divided into control, model, EA, and MLA groups.
Induced colitis using 2.5% dextran sodium sulfate for 7 days.
Assessed colitis severity via body weight, colon length, and histological analysis.
Measured pro-inflammatory cytokines using MSD assay.
Recorded vagal efferent fiber activity electrophysiologically.
EA at ST37 reduced body weight loss and colon damage (P < 0.05).
Cytokine levels decreased after EA treatment (P < 0.05).
ChAT-positive neuron activation in the DMV increased with EA (P < 0.05).
Restored α 7nAChR levels in the proximal colon after EA (P < 0.05).
Enhanced activation of cervical vagal efferent fibers post-EA (P < 0.05).

Abstract

Background: Colitis impairs life quality, and treatments are limited. Acupuncture may engage vagal anti-inflammatory pathways, but the precise circuits are unclear. Objective: This study aimed to examine the effects of electroacupuncture (EA) at “Shangjuxu” (ST37) on murine colitis and the involvement of the cholinergic vagus system. Methods: Mice were divided into control, model, EA (electro-acupuncture), and MLA (EA with α 7nAChR antagonist) groups. A colitis model was induced in mice by freely drinking a 2.5% dextran sodium sulfate (DSS) solution for 7 days. Colitis severity was assessed via body weight, colon length, and H&E staining. Proximal colon cytokines were measured by MSD assay. Immunofluorescence evaluated activation of ChAT-positive neurons in the dorsal motor nucleus of the vagus (DMV) and local colon cholinergic markers. Anatomical interactions between ChAT-positive fibers and α 7nAChR-positive cells were observed. Vagal efferent fiber activity was recorded electrophysiologically. Results: EA at ST37 significantly reduced body weight loss and proximal colon tissue damage in colitis mice (P < 0.05). These beneficial effects were blocked by the α 7nAChR antagonist MLA. Levels of five pro-inflammatory cytokines (TNF-α, IL-6, CXCL1, IL-1β, IL-5) were elevated in the model group but decreased following EA treatment (P < 0.05). Colitis increased activation of ChAT-positive neurons in the DMV, and EA further enhanced this activation (P < 0.05). The reduced α 7nAChR content in the proximal colon of model mice was restored to near-normal levels by EA (P < 0.05). EA also significantly increased the activation of cervical vagal efferent fibers (P < 0.05). Anatomical associations between ChAT-positive fibers and α 7nAChR-positive cells were observed in the EA group. Conclusion: EA at ST37 alleviates inflammation in colitis mice likely by activating ChAT-positive neurons in the DMV. This stimulates vagal efferent nerves, modulates α 7nAChR-positive cells in the proximal colon, and engages the vagal cholinergic anti-inflammatory pathway. Keywords: electroacupuncture, vagus nerve, ulcerative colitis, neuroimmune regulation, cholinergic anti-inflammatory

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