Metabolomic profiling of the leaves, floral buds, flowers, and fruits of Ternstroemia lineata was conducted across the dry and rainy seasons in Mexico. The presence of katsumadin was determined and its antibacterial activity was evaluated. Multivariate data analysis was performed using proton nuclear magnetic resonance (1H NMR) and Principal Component Analysis (PCA). Katsumadin was identified by 1H NMR and, quantified by HPLC, and its antibacterial activity was assessed using a microdilution assay. The results revealed conserved 1H NMR signals among leaf, flower buds, flowers and fruits, as well as signals that were either organ-specific or time-dependent. The spatial and temporal distribution of katsumadin was monitored over a nine-month period. During the dry season, katsumadin reached 1.5 mg/g DW in the leaves, whereas it was not detected during the rainy season. Higher katsumadin contents were observed in floral buds, fruits, and flowers (6.09 ± 0.10, 3.0 ± 0.79, and 3.4 ± 0.42 mg/g DW, respectively). In addition, katsumadin was evaluated against Salmonella Typhi and Pseudomonas aeruginosa, exhibiting a minimum inhibitory concentration and bactericidal effect at 0.5 mg/mL.
Soto-Díaz et al. (Fri,) studied this question.