italicWenyang Shengji Ointment/italic（温阳生肌膏） alleviates inflammation in diabetic wounds via the p38 MAPK/NF-B signaling pathway

Key Points

• The aim is to explore how Wenyang Shengji Ointment modulates the p38 MAPK/NF-κB signaling pathway to mitigate inflammation in a high glucose environment.
• Established high glucose conditions using RAW264.7 and NIH3T3 cell lines.
• Divided into normal, model, and Wenyang Shengji Ointment treatment groups.
• Assessed cell proliferation, migration, macrophage polarization, TNF-α levels, and protein expression via various assays including CCK-8, scratch assay, flow cytometry, ELISA, and Western Blot.
• Wenyang Shengji Ointment significantly promotes cell proliferation (P<0.01 or P<0.001).
• It inhibits RAW264.7 macrophages from polarizing to M1 type (P<0.001) while promoting M2 type polarization (P<0.001).
• The ointment restores migration ability in NIH3T3 and co-cultured cells (P<0.01).
• It reduces the expression levels of p38 MAPK and NF-κB p65 proteins in NIH3T3 cells (P<0.05).
• It significantly lowers TNF-α levels and signals related to inflammation (P<0.001).