Targeted Detection Method for Locus-specific m⁶A Modifications in Low-Abundance Transcripts Based on Chemical Conversion

Key Points

• The aim is to develop a sensitive method for detecting locus-specific m6A modifications in low-abundance transcripts.
• Utilized sodium nitrite and glyoxal for chemical conversion
• Employed probe hybridization to detect m6A modifications
• Focused on enhancing sensitivity for low-abundance transcripts
• Achieved highly sensitive detection of target m6A modifications
• Demonstrated effective locus-specific targeting in transcript analysis