(1) Brucellosis is a zoonotic infection that remains a significant public health concern in endemic regions. This study aimed to determine the seroprevalence of brucellosis in a tertiary care hospital, analyze associated risk factors, and evaluate the diagnostic performance of commonly used serological tests. (2) The study was based on the serological test results of 24,545 samples collected between 2020 and 2023. Rose Bengal, standard tube agglutination, and Brucellacapt tests were used for the diagnosis of brucellosis. Data were analyzed according to age, sex, clinical department, and seasonal distribution using SPSS version 25.0. (3) Overall, 367 cases (1.5%) tested positive. When the 367 seropositive cases were evaluated by year, the annual distribution showed a declining trend, decreasing from 2.5% in 2020 to 1.2% in 2023. Among the positive cases, 57.8% were female, and 36% were aged between 41 and 64 years. The infectious diseases department had the highest positivity rate (37.1%). Brucellacapt showed the highest positivity rate (90.2%), followed by Rose Bengal (76.2%). The highest monthly positivity rate was observed in October (11.4%), and seasonally in autumn (31.3%). (4) The Brucellacapt test has demonstrated high sensitivity and serves as a valuable supplementary diagnostic tool in the evaluation of brucellosis. However, its low specificity underscores the necessity for careful interpretation of positive results and supports its use in conjunction with other serological tests to enhance diagnostic accuracy. Considering seasonal and departmental variations, a combined testing approach may improve overall diagnostic accuracy.
Kirisci et al. (Sat,) studied this question.