The application of nitrification inhibitors (Nis) with nitrogen fertilizers is increasingly used as a management strategy to improve nitrogen use efficiency in crop production systems. To evaluate the effects of Ni dicyandiamide (DCD) and 1,2,4-triazole (TZ) on the rhizosphere microbiome and strawberry yield (Fragaria × ananassa Duch.), a two-year field experiment was conducted with three treatments: unfertilized control (C), mineral nitrogen fertilizer (N) applied in two doses (40 + 40 kg N ha−1 year−1), and a single nitrogen application (80 kg N ha−1 year−1) combined with nitrification inhibitors (N + Ni). Soil microbiota were assessed using cultivation-based methods and metabarcoding of 16S rRNA and ITS2 regions. Total bacterial counts on complex media increased from 5.85 to 6.15 log CFU g−1 in the N treatment, while remaining 5.89 in N + Ni. Microscopic fungi increased in fertilized treatments during spring but decreased in July of the second year. Microbial community composition differed among treatments, although sampling time explained a larger proportion of variability than fertilization. Relative abundance of Gemmatimonas decreased under N + Ni, whereas Nitrososphaera increased. Fungal Shannon diversity decreased in N + Ni, while prokaryotic diversity did not differ significantly. Despite similar levels of mineral nitrogen measured before harvest, strawberry yield increased significantly in the N + Ni treatment in the second year, reaching 109% higher values than the control and 80% higher than the N treatment. This may indicate that the fertilization regime including nitrification inhibitors influenced nitrogen availability earlier in the growing season.
Maková et al. (Mon,) studied this question.