Aqueous Extract of Pursane (Portulaca Oleracea) on Growth Performance, Serum, Liver and Mucus Immunity, and Inflammatory Factor Gene Expression in Common Carp (Cyprinus Carpio)

This experiment was conducted to investigate the effects of dietary supplementation with Aqueous Extract of Portulaca oleracea (AEP) on growth performance, serum, liver and mucus immunity, and inflammatory factor gene expression in immune organs of common carp (Cyprinus carpio). A total of 240 juvenile carp (32.60±0.31) g were randomly divided into four dietary groups with three replicates in each group and 20 carps in each replicate. The control group was fed with basal diet without AEP, and the experimental groups were fed with basal diet supplemented with 0.25%, 0.50% and 1.00% AEP for 8 weeks, respectively. Results demonstrated the following key findings: (1) Compared with the control group, the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed coefficient (FCR), and feeding rate (FR) were not significantly affected (P>0.05). However, the viscerosomatic index (VSI) was significantly decreased and the condition factor (CF) was significantly increased (PPPPPPPP>0.05). (5) The mRNA expression analysis revealed that TNF-α and TGF-β levels in spleen tissue were downregulated in the 0.25% and 0.50% AEP groups (PTNF-α was also reduced in the 1.00% AEP group (PIL-1β expression was significantly down-regulated across all AEP treatments, while IL-10 was significantly reduced in 0.50% and 1.00% AEP groups (P0.05). In conclusion, dietary AEP supplementation enhance the immune function in serum, liver, and mucus of common carp without affecting growth performance. It also modulates the gene expression of inflammatory factors in immune organs, suggesting a potentially beneficial role in immune response process of the organism.