ABSTRACT High ESBL prevalence in MDR E. coli and Klebsiella from clinical and wastewater samples, with concerning AmpC and carbapenemase occurrence and frequent co-resistance. Multidrug-resistant (MDR) gram-negative bacteria pose a global health threat, with MDR-Escherichia coli and MDR-Klebsiella pneumoniae as the primary contributors among Enterobacterales. This study explored the prevalence of extended-spectrum beta-lactamase (ESBL), AmpC-β-lactamase and carbapenemase-encoding genes among ESBL-producing E. coli and Klebsiella spp. isolated from clinical and hospital wastewater samples in Burkina Faso. We analyzed 292 ESBL-producing E. coli and Klebsiella spp. isolates from clinical specimens (n = 218) and hospital wastewaters (n = 74) across five Burkinabe hospitals. Using the conventional PCR multiplex technique, we screened for 10 key resistance genes, covering ESBL (blaCTX-M, blaSHV and blaTEM), AmpC-β-lactamase (blaDHA and blaFOX) or carbapenemases (blaOXA-48, blaNDM, blaVIM, blaKPC and blaIMP). Resistance genes were present in both clinical and wastewater samples across all levels of healthcare facilities. ESBL-encoding genes were detected in 95.5% of the isolates, AmpC-β-lactamase genes in 11.0% and carbapenemase-encoding genes in 17.1%. Many isolates from both clinical and hospital wastewater samples harbored multiple resistance genes, spanning all three gene categories. The findings from this study underscore the urgent need for a comprehensive hospital-based AMR surveillance system and stringent antibiotic stewardship measures to limit the spread of resistant bacteria in the environment to protect public health.
Garba et al. (Fri,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: