The extraction of secondary metabolites was carried out by using water, a mix of methanol and water (8:2) with NaF, as well as methanol, ethanol, and acetone (all mixed with water in a 7:3 ratio) from various parts (like leaves, flowers, stems, and roots) of Physalisperuviana L.(Cape Gooseberry) and Phyllanthus emblicand (Amala) with the help of decoction and maceration methods. This paper states that methanol provides the best results for decoction, while acetone worked best for maceration. The total polyphenol content (TPC) achieved through decoction showed the highest TPC levels, and MeOH with NaF was found to be the most effective solution for extracting TPC. Maceration proved better for extracting flavonoids, with ethanol and acetone being the most effective solvents. In general, the highest levels of TPC and flavonoids were obtained from leaves and fruits of Physalisperuviana L. (Cape Gooseberry) and Phyllanthus (Amala) regardless of the solvent or extraction method applied. Furthermore, the roots of Physalisperuviana L.(Cape Gooseberry) and Phyllanthus emblicand (Amala) showed important levels of these compounds in consonance with the total antioxidant activity (TAA) evaluated in the different organs of the plant in the these species. In this study, the solvents and extraction methods applied were tools that determined significantly the level of extraction of bioactive compounds, showing a different impact on plant organs for each medicinal species studied.
*Anil Kumar (Sat,) studied this question.
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