Background: Eggshell membrane (ESM) is a natural biomaterial rich in collagen, elastin, glycosaminoglycans, and hyaluronic acid. These bioactive components are fundamental to skin structure and have shown potential for improving skin health when administered orally. However, clinical evidence supporting the anti-aging effects of oral ESM supplementation remains limited. Objective: To evaluate the effects of oral ESM supplementation on skin biomechanical properties, wrinkle parameters, surface roughness, and self-perceived skin quality in middle-aged women over a 90-day treatment period. Methods: A single-arm clinical study was conducted in 33 female volunteers (age: 38–66 years; mean 51.5 ± 8.2) with moderate-to-severe signs of aging. Participants received 500 mg/day of soluble ESM orally for 90 days. Skin assessments were performed at baseline (D0), Day 45 (D45), and Day 90 (D90) using 3D fringe projection analysis (AEVA-HE) for wrinkle and surface roughness parameters, and cutometry (Cutometer MPA Dual 580) for biomechanical properties. A self-assessment questionnaire was administered at each time point. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Results: Oral ESM supplementation significantly improved skin distensibility (R0), with increases of 6.8% at D45 (p 0.05) and 9.6% at D90 (p 0.05) compared to baseline. Wrinkle parameters and surface roughness remained stable throughout the treatment period, with no statistically significant deterioration. Self-perception assessments revealed that 75.8% of participants reported smoother expression lines, 66.7% perceived improved hydration, and 60.6% noted enhanced firmness and elasticity at D90. The treatment demonstrated excellent skin compatibility with no serious adverse effects. Conclusions: Daily oral supplementation with 500 mg of soluble ESM for 90 days significantly improved skin distensibility and was associated with favorable self-perceived improvements in multiple skin quality parameters. These findings support the potential of oral ESM as a well-tolerated nutraceutical approach for skin health maintenance in aging women.
Javier Moran (Tue,) studied this question.