x-Ray Structure of Streptomyces avermitilis Phospholipase D Reveals a Ca2+-Stabilized Expanded Active-Site Cleft Adapted for Phospholipid Binding.

Key Points

• The research aims to understand the molecular structure of phospholipase D and its binding affinity for phospholipids.
• Conducted X-ray crystallography to determine the structure of phospholipase D from Streptomyces avermitilis.
• Analyzed the active-site cleft and identified stabilization mechanisms by calcium ions.
• Characterized phospholipid specificity through structural insights.
• The expanded active-site cleft stabilizes phospholipid binding through calcium ion interactions.
• Structural data revealed key mechanisms underlying specificity for different phospholipid types.