Abstract Background: Recurrent miscarriage is characterized by two or more spontaneous abortions and can be attributed to various factors, including anatomy, endocrinology, genetics, immunology, and microbial infections, although the exact cause remains unclear in up to 50% of cases, with the genetic background of the host, cytomegalovirus (CMV) infections, and interleukin-6 (IL-6) single nucleotide polymorphisms being of significant importance. Objectives: Particular primers were used to study single nucleotide polymorphisms (-174 r1800795 G/C) in the IL-6 gene in order to assess the correlation between CMV infection and recurrent pregnancy loss (RPL) vulnerability through the employment of tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Materials and Methods: Fifty female subjects diagnosed with recurrent pregnancy loss and cytomegalovirus infection were selected as the case group, whereas the control group consisted of 50 healthy individuals who had given birth without CMV infection; blood samples were collected from September 2022 to January 2023. DNA was extracted to study single nucleotide polymorphisms in the IL-6 gene. Results: The PCR analysis yielded results that indicate the presence of G and C alleles, with three distinct genotypes (GG, GC, and CC) and varying lengths (302, 206, and 152) bp. Additionally, the stratified analysis revealed a strong and significant association between the patient and control groups. Conclusion: Potential association between the rs1800795 G>C polymorphism in IL-6 gene and RPL susceptibility.
Alsaikel et al. (Thu,) studied this question.