At low concentrations, quercetin significantly reduced the cytotoxicity, oxidative stress, and apoptosis induction of doxorubicin in SW480 colorectal cancer cells (P<0.05).
Does quercetin affect the cytotoxicity, oxidative stress, and apoptosis induced by doxorubicin in SW480 colorectal cancer cells?
Quercetin supplementation at low concentrations may decrease the therapeutic efficacy of doxorubicin in colorectal cancer cells by lowering oxidative stress damage, potentially leading to chemoresistance.
p-value: p=<0.05
Abstract Background: Quercetin is one of the important flavonoids that have antioxidant/pro-oxidant properties and apoptotic impact on cancer cells. Doxorubicin is a DNA intercalating agent that is utilized to manage a variety of cancers. Cardiotoxicity limits the usage of doxorubicin despite the fact that it is powerful in the treatment of cancer. Objectives: To evaluate the impact of quercetin on oxidative stress, apoptosis, and its effect on the therapeutic cytotoxic profile of doxorubicin in SW480 colorectal cancer cell lines. Materials and Methods: SW480 cancer cells and Vero (nontumoral) cells were provided by the Tissue Culture Laboratory in the College of Medicine, University of Babylon. Assessment of the safety of quercetin concentrations on Vero cells by MTT assay to be then selected in combination with IC50 of doxorubicin. Anticancer effects of each of quercetin and doxorubicin alone and in combination on SW480 cells were evaluated using MTT assay and enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay analysis of glutathione (GSH), malondialdehyde (MDA) and caspase 3 levels. Results: Vero cells, in a 48-h period, had cell viability of 81.27% and 75% at high concentrations of quercetin (100 and 200 µM), respectively. However, cell viability was 98.12% and 96.86% when Vero cells were incubated with 25 and 50 µM, respectively. Each one of quercetin and doxorubicin significantly (* P < 0.05) and (** P < 0.001) inhibited cell proliferation and induced apoptosis by increasing the level of oxidative stress in SW480 cells in a dose-dependent manner. At low concentrations, quercetin significantly reduced the cytotoxicity, oxidative stress, and apoptosis induction of doxorubicin. Conclusion: Using quercetin at low concentrations decreases the therapeutic impacts of doxorubicin by lowering oxidative stress damage. Quercetin supplementation while receiving chemotherapy can result in resistance to chemotherapies.
Al-Nassrawi et al. (Thu,) conducted a other in Colorectal cancer. Quercetin and doxorubicin vs. Quercetin alone, doxorubicin alone, or in combination was evaluated on Cell proliferation, oxidative stress (GSH, MDA), and apoptosis (caspase 3 levels) (p=<0.05). At low concentrations, quercetin significantly reduced the cytotoxicity, oxidative stress, and apoptosis induction of doxorubicin in SW480 colorectal cancer cells (P<0.05).