, IGAS enabled real-time monitoring of intracellular ATP fluctuations throughout the bacterial growth cycle, demonstrating high consistency with standard luciferase assays. Furthermore, guided by molecular dynamics (MD) simulations, we identified key residues to engineer IGAS variants with tunable affinities. These sensors were successfully applied to diverse cellular environments, ranging from cytoplasmic targeting to mammalian cell surface display. Collectively, our results demonstrate the excellent reversibility and versatility of IGAS, establishing it as a powerful tool for dynamic ATP detection in complex biological systems.
Deng et al. (Wed,) studied this question.
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