What does this research mean for the field?

A single-round, multiplex PCR targeting the conserved 18S small-subunit RNA genes can rapidly detect and differentiate Plasmodium falciparum, P. vivax, P. malariae, and P. ovale in human blood, even in cases of mixed infection. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

March 1, 2003

Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present

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Abstract

A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.

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