Sortase A-Mediated Farnesylation of Cdc42 In Vitro

Key Points

• The study aims to investigate the farnesylation of Cdc42 mediated by sortase A and its subsequent functionality.
• Cdc42 was expressed with a sortase A recognition motif.
• Farnesylation and purification were achieved using a size exclusion strategy.
• Functional assays included assessments of membrane binding and GEF-regulatory GTPase activity.
• Farnesylated Cdc42 demonstrated effective membrane binding.
• Farnesylated Cdc42 retained GEF-regulatory GTPase activity.