This dataset contains the source data supporting the findings reported in Lathan, Kaya et al. , Nature Communications (2025), "Wavelength-Dependent Feedback Behaviour in Light-Gated Polymersome Nanoreactors. " The study describes the design and characterisation of synthetic polymersome nanoreactors functionalised with a broad-spectrum, pyrazolone-based Donor–Acceptor Stenhouse Adduct (DASA) photoswitch (PYRA) and encapsulating porcine liver esterase. These PYRA–esterase nanoreactors exhibit wavelength-dependent, orthogonal positive and negative feedback loops: irradiation with yellow light (590 nm) produces an autocatalytic positive feedback loop mediated by spectral unmasking of the photoswitch, while irradiation with blue light (405 nm) generates a self-limiting negative feedback loop through competitive light absorption by the pH-sensitive dye bromocresol purple (BPH). The dataset includes the raw and processed data underlying all main-text figures (Figures 1–4) and supplementary figures (Supplementary Figures 1–16), encompassing: UV–vis absorbance time-course data for ethyl acetate hydrolysis experiments under yellow and blue light irradiation at varying intensities Photoisomerization kinetics of the PYRA polymer in THF and aqueous dispersions under alternating light/dark cycles Dynamic light scattering (DLS) measurements of PYRA–esterase nanoreactors before and after light exposure Gel permeation chromatography (GPC) traces for PYRA polymer characterisation ATR FT-IR spectra from stepwise polymer synthesis ¹H NMR data used for block copolymer composition analysis Cryo-TEM image data for nanoreactor morphology characterisation under different conditions (dark, yellow light, blue light, pH cycling) Absorbance–pH calibration curve data for bromocresol purple Feedback reversal and sequential orthogonal irradiation experiments (rate-of-change data) Comparative hydrolysis data for alternative ester substrates (phenyl acetate, ethyl propionate) Methods summary: Nanoreactors were assembled by solvent-exchange self-assembly of a PEG-b-PHMA diblock copolymer functionalised with a third-generation pyrazolone DASA, with simultaneous esterase encapsulation and subsequent trypsinisation to remove non-encapsulated enzyme. Enzymatic activity was monitored by UV–vis spectroscopy using a 96-well plate reader (Molecular Devices SpectraMax M5). Particle size and morphology were assessed by DLS (Malvern Zetasizer Ultra) and cryo-TEM (Talos F200C, 120 kV). Related publication: Lathan U†, Kaya B†, Matubbar F, Chami M, Yeow J, Rifaie-Graham O. Wavelength-Dependent Feedback Behaviour in Light-Gated Polymersome Nanoreactors. Nature Communications (2025). †Equal contribution. Corresponding author: Omar Rifaie-Graham, Department of Chemistry, Queen Mary University of London (o. rifaiegraham@qmul. ac. uk) Funding: Queen Mary University of London; The Royal Society (Research Grant RG1\251346)
Lathan et al. (Tue,) studied this question.