Conscious sinoaortic baroreceptor-denervated rats allow modeling of renal blood flow autoregulation, showing furosemide shifts the lower limit of autoregulation rightward to 102 +/- 5 mmHg.
The conscious SAD rat model allows for the description of renal blood flow autoregulation curves under dynamic conditions, demonstrating that tubuloglomerular feedback and myogenic mechanisms cooperate to stabilize RBF.
These experiments examined whether the conscious sinoaortic baroreceptor-denervated (SAD) rat, owing to its high spontaneous arterial pressure (AP) variability, might represent a model for renal blood flow (RBF) autoregulation studies. In eight SAD and six baroreceptor-intact rats, AP and RBF were recorded (1-h periods) before and after furosemide (10 mg/kg followed by 10 mg. kg(-1). h(-1) iv) administration. In control conditions, AP variability was markedly enhanced in SAD rats (coefficient of variation: 16.0 +/- 1.2 vs. 5.4 +/- 0.5% in intact rats), whereas RBF variability was only slightly increased (8.7 +/- 0.6 vs. 6.1 +/- 0.5% in intact rats), suggesting buffering by autoregulatory mechanisms. In SAD rats, but not in intact rats, the AP-RBF relationships could be modeled with a four-parameter sigmoid Weibull equation (r(2) = 0.24 +/- 0.07, 3,600 data pairs/rat), allowing for estimation of an autoregulatory plateau (10.1 +/- 0.7 ml/min) and a lower limit of RBF autoregulation (P(LL) = 93 +/- 6 mmHg, defined as AP at RBF 5% below the plateau). After furosemide treatment, autoregulation curves (r(2) = 0.49 +/- 0.07) in SAD rats were shifted downward (plateau = 8.6 +/- 0.8 ml/min) and rightward (P(LL) = 102 +/- 5 mmHg). In five of six intact rats, P(LL) became measurable (104 +/- 1 mmHg), albeit with limited accuracy (r(2) = 0.09 +/- 0.03). In conclusion, the conscious SAD rat offers the possibility of describing RBF autoregulation curves under dynamic, unforced conditions. The tubuloglomerular feedback and myogenic mechanisms cooperate in setting P(LL) and thus in stabilizing RBF during spontaneous depressor episodes.
Pires et al. (Tue,) conducted a other in Sinoaortic baroreceptor denervation (animal model) (n=14). Furosemide vs. Before furosemide administration / baroreceptor-intact rats was evaluated on Lower limit of renal blood flow autoregulation (P(LL)). Conscious sinoaortic baroreceptor-denervated rats allow modeling of renal blood flow autoregulation, showing furosemide shifts the lower limit of autoregulation rightward to 102 +/- 5 mmHg.