In vitro experiments demonstrated that stainless steel stents can be successfully seeded with human endothelial cells, and in vivo porcine implantations showed complete natural endothelial coverage of stent wires within 6 days.
Does seeding intravascular stents with endothelial cells result in successful endothelialization in a porcine model?
Seeding stents with endothelial cells prior to implantation results in complete endothelial coverage within 1 week in a porcine model, suggesting a potential strategy to protect against early thrombosis.
Wide clinical application of intravascular stenting devices is currently limited by occlusion or intraluminal narrowing caused by thrombosis and neointimal thickening in a considerable percentage of implantations. We studied the possibility of seeding one of the currently availiable stents, a stainless steel, self‐expandable wire‐mesh, with endothelial cells in vitro. Endothelial cells, derived from human umbilical cord veins, could be successfully attached to stent filaments. In vivo stent implantations in porcine femoral arteries showed complete covering of stent wires by endothelium after 1 week. We conclude that coating of stents with autologous endothelial cells prior to implantation might protect against early thrombosis during the period in which a neointima is formed. (J Interven Cardiol 1988:1:2)
Giessen et al. (Wed,) conducted a other in Intravascular stenting (n=6). Intravascular stent implantation (in vivo) and endothelial cell seeding (in vitro) was evaluated on Endothelial coverage and stent patency. In vitro experiments demonstrated that stainless steel stents can be successfully seeded with human endothelial cells, and in vivo porcine implantations showed complete natural endothelial coverage of stent wires within 6 days.
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