November 1, 1989Open Access
Biosynthesis of titin in cultured skeletal muscle cells.
Structured PICO
P
Population
Synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos
I
Intervention
Pulse-labeling with [35S]methionine and immunoprecipitation with an anti-titin mAb
O
Outcome
Biosynthesis rate, half-life, and extraction resistance of titinsurrogate
Newly synthesized titin molecules in developing skeletal muscle cells are stable proteins that rapidly associate with the cytoskeleton.
Abstract
Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with 35Smethionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases greater than 4-fold during the first week in culture and during this same time period, synthesis of muscle-specific myosin heavy chain increases greater than 12-fold; (b) newly synthesized titin has a t1/2 of approximately 70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.