Low temperature and low irradiance (LTLI) stress severely limits strawberry growth and productivity during winter protected cultivation. This study investigated the physiological responses of the short-day strawberry cultivar ‘Benihoppe’ to individual and combined LTLI stress and developed a quantitative damage evaluation index. Seedlings were exposed to four treatments for 20 d: control (25/15 °C, 600 μmol m−2 s−1), single low temperature (LT: 15/5 °C), single low irradiance (LI: 100 μmol m−2 s−1), and combined stress (LTLI: 15/5 °C, 100 μmol m−2 s−1). Compared to isolated stress factors, combined LTLI treatment exhibited a statistically verified synergistic damaging effect (two-factor ANOVA, LT × LI p 0.9) with key stress markers (Fv/Fm, Pn, and MDA). Overall, combined LTLI stress exacerbates structural degradation and PSII photoinhibition in strawberry leaves. The proposed LTLDI offers a practical, standardized tool for evaluating stress severity, facilitating timely environmental management in greenhouse strawberry production.
Xu et al. (Fri,) studied this question.
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