Key points are not available for this paper at this time.
SummaryUsing the criteria of generation time, final population density, and chromosome analysis, it has been demonstrated that Eagle basal medium is adequate for continued propagation of L-M strain mouse cells both as monolayers and in suspension. Three distinct advantages to this system are: (1) the relative simplicity of the medium in terms of number of components as compared to other defined media currently available, (2) the 3- to 5-fold increase in cell population achieved in 7 to 10 days, in contrast with results using most defined media, (3) the growth of these cells in suspension culture, which is an ideal system for both nutritional and parasite-host cell relationship studies. The effectiveness of methylcellulose as a physical protective agent for cells grown in a simple, chemically defined medium has been demonstrated.
Merchant et al. (Tue,) studied this question.