Time-lapse imaging reveals that cell-cell contact triggers desmoplakin assembly into nascent junctions from diffusible and particulate pools, regulated by actin and intermediate filament interactions.
Desmoplakin assembles into nascent junctions from diffusible and particulate pools in a series of events triggered by cell-cell contact and regulated by actin and DP-IF interactions.
The intermediate filament (IF)-binding protein desmoplakin (DP) is essential for desmosome function and tissue integrity, but its role in junction assembly is poorly understood. Using time-lapse imaging, we show that cell-cell contact triggers three temporally overlapping phases of DP-GFP dynamics: (1) the de novo appearance of punctate fluorescence at new contact zones after as little as 3 min; (2) the coalescence of DP and the armadillo protein plakophilin 2 into discrete cytoplasmic particles after as little as 15 min; and (3) the cytochalasin-sensitive translocation of cytoplasmic particles to maturing borders, with kinetics ranging from 0.002 to 0.04 microm/s. DP mutants that abrogate or enhance association with IFs exhibit delayed incorporation into junctions, altering particle trajectory or increasing particle pause times, respectively. Our data are consistent with the idea that DP assembles into nascent junctions from both diffusible and particulate pools in a temporally overlapping series of events triggered by cell-cell contact and regulated by actin and DP-IF interactions.
Godsel et al. (Mon,) reported a other. Time-lapse imaging of DP-GFP dynamics was evaluated on Desmoplakin assembly dynamics. Time-lapse imaging reveals that cell-cell contact triggers desmoplakin assembly into nascent junctions from diffusible and particulate pools, regulated by actin and intermediate filament interactions.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: