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PURPOSE: The aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene B(e)P-induced toxicity in ARPE-19 cells. METHODS: We pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured. RESULTS: < 0.05). CONCLUSION: In conclusion, our results demonstrate that 17β-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential.
Kenney et al. (Mon,) studied this question.
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