What question did this study set out to answer?

The central aim is to explore the mechanism by which WTHXC treats diabetic peripheral neuropathy through the IRE1α/JNK/Caspase3 signaling pathway.

June 3, 2026

温通活血乳膏通过IRE1/JNK/Caspase3信号通路改善糖尿病周围神经病变的机制研究

Key Points

The central aim is to explore the mechanism by which WTHXC treats diabetic peripheral neuropathy through the IRE1α/JNK/Caspase3 signaling pathway.
70 SD male rats were divided into treatment groups, with WTHXC applied for 4 weeks.
Body weight, blood glucose, and latencies were measured, along with histological analysis and apoptosis detection.
In vitro studies on RSC96 cells assessed the impact of WTHXC and IRE1α inhibitors on apoptosis and gene expression.
WTHXC significantly improved tail flick latency and reduced apoptosis, lowering p-IRE1α and Caspase3 protein levels (P<0.01).
The high-dose WTHXC group showed better outcomes than the low-dose group.
In vitro, WTHXC decreased apoptosis rates and expression of IRE1α, p-JNK, and Caspase3 (P<0.05 or P<0.01).

Abstract

目的基于IRE1α/JNK/Caspase3信号通路，探讨温通活血乳膏（WTHXC）治疗糖尿病周围神经病变（DPN）的作用机制。方法 70只SD雄性大鼠，随机选取10只为空白组（普通饮食），其余60只使用小剂量STZ联合高糖高脂饮食喂养制备成为DPN模型，成模后分为模型组、WTHXC高剂量组、WTHXC低剂量组、WTHXC基质组、二甲双胍组，每组12只，药物治疗组分别给予WTHXC高、低剂量及基质涂抹双后肢，二甲双胍灌胃，给药4周。检测大鼠体质量、血糖、热甩尾潜伏期，HE染色观察坐骨神经病理，TUNEL检测细胞凋亡，Western blot和RT-PCR检测IRE1α/JNK/Caspase3信号通路相关蛋白及基因表达。体外将RSC96细胞分为空白组、模型组、WTHXC组，IRE1α抑制剂组、WTHXC+IRE1α抑制剂组，高糖培养构建模型，给予对应干预，检测细胞凋亡及相关蛋白、基因表达。结果温通活血乳膏可改善热甩尾潜伏期及坐骨神经病理，减少凋亡，降低p-IRE1α、Caspase3蛋白表达（PPP0.05或P 结论 WTHXC通过IRE1α/JNK/Caspase3信号通路抑制坐骨神经雪旺细胞凋亡，恢复坐骨神经结构完整性，从而起到对DPN的治疗作用。

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温通活血乳膏通过IRE1/JNK/Caspase3信号通路改善糖尿病周围神经病变的机制研究

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