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Biochemical and functional studies of ion channels have shown that many of these integral membrane proteins form macromolecular signaling complexes by physically associating with many other proteins. These macromolecular signaling complexes ensure specificity and proper rates of signal transduction. The large-conductance, Ca 2+ -activated K + (BK) channel is dually activated by membrane depolarization and increases in intracellular free Ca 2+ (Ca 2+ i ). The activation of BK channels results in a large K + efflux and, consequently, rapid membrane repolarization and closing of the voltage-dependent Ca 2+ -permeable channels to limit further increases in Ca 2+ i . Therefore, BK channel-mediated K + signaling is a negative feedback regulator of both membrane potential and Ca 2+ i and plays important roles in many physiological processes and diseases. However, the BK channel formed by the pore-forming and voltage- and Ca 2+ -sensing α subunit alone requires high Ca 2+ i levels for channel activation under physiological voltage conditions. Thus, most native BK channels are believed to co-localize with Ca 2+ -permeable channels within nanodomains (a few tens of nanometers in distance) to detect high levels of Ca 2+ i around the open pores of Ca 2+ -permeable channels. Over the last two decades, advancement in research on the BK channel’s coupling with Ca 2+ -permeable channels including recent reports involving NMDA receptors demonstrate exemplary models of nanodomain structural and functional coupling among ion channels for efficient signal transduction and negative feedback regulation. We hereby review our current understanding regarding the structural and functional coupling of BK channels with different Ca 2+ -permeable channels.
Shah et al. (Fri,) studied this question.