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April 18, 1967Proceedings of the Royal Society of London. Series B, Biological sciences432 citations

On the conformation of the hen egg-white lysozyme molecule

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CBC. C. F. BlakeGMG. A. MairANA.C.T. North

Key Points

  • The study aims to determine the conformation of hen egg-white lysozyme crystals.
  • Crystals were grown at pH 4.7 and analyzed using X-ray diffraction methods.
  • Isomorphous replacement and anomalous scattering were utilized to derive phase information.
  • Heavy atom derivatives were tested to produce an electron density map at 2 Å resolution.
  • Electron density map calculated with a minimum r.m.s. error shows detailed structure of lysozyme.
  • Three heavy atom derivatives successfully calculated the electron density, enhancing crystal structure understanding.

Abstract

Abstract Crystals of hen egg-white lysozyme, grown at pH 4.7 (Alderton Blake, Fenn, North, Phillips Blow 1958; Perutz, Rossmann, Cullis, Muirhead, Will Blake, Koenig, Mair, North, Phillips & Sarma 1965) and three proved satisfactory for calculating an electron density map at 2 Å resolution. They contained respectively ortho-mercuri hydroxytoluene para-sulphonic acid, UO2F53- and an ion derived from UO2(NO3)2, probably UO2(OH)n(n-2)-

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Cite This Study

Blake et al. (1967) studied this question.

synapsesocial.com/papers/6a2082cc23a8c2fe81f893f1https://doi.org/10.1098/rspb.1967.0034
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