Ultra-performance convergence chromatography tandem mass spectrometry strategy for quantification of monodisperse polyethylene glycol polymers coupled with multiple fragmentation to enhance sensitivity

Key Points

• The aim is to accurately quantify monodisperse polyethylene glycol derivatives in complex biological matrices, overcoming challenges of matrix interference.
• Utilized ultra-performance convergence chromatography coupled with tandem mass spectrometry for analysis.
• Implemented multiple fragmentation techniques to enhance sensitivity.
• Conducted testing on complex biological matrices to evaluate retention and quantification accuracy.
• Demonstrated improved sensitivity and accuracy in detecting monodisperse polyethylene glycol derivatives.
• Achieved lower limits of detection compared to conventional methods, indicating better performance in complex samples.
• Highlighted significant reductions in matrix interference, enhancing overall quantification reliability.