Population
Preclinical models including C57BL/6 mice, various knockout mice, murine L929 fibroblasts, primary murine…
Comparison
Infection with EndoU-deficient coronaviruses vs Infection with wild-type coronaviruses or mock…
Design
Preclinical
Key result
EndoU-deficient coronaviruses are severely attenuated in vivo and in primary cells due to early induction of double-stranded RNA host cell responses, including IFN-I expression and RNase L activation.
Authors
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May guide antiviral targeting of EndoU; hypothesis-generating from animal models only.
Coronavirus endonuclease (EndoU) activity is a critical evolutionary conserved mechanism that allows the virus to evade early host innate immune responses by preventing the simultaneous activation of dsRNA sensors like Mda5, OAS, and PKR.
Kindler et al. (2017) studied Coronavirus infection. EndoU-deficient coronaviruses vs. Wild-type coronaviruses was evaluated on Viral replication and innate immune response. EndoU-deficient coronaviruses are severely attenuated in vivo and in primary cells due to early induction of double-stranded RNA host cell responses, including IFN-I expression and RNase L activation.