Engineered Schemes of Directed Cellular Evolution for Aging Intervention

This paper reviews cutting-edge technologies in the field of aging intervention and proposes an aging intervention program utilizing existing technologies. Instead of repairing aging hallmarks one by one, the program leverages cells’ inherent evolutionary capacity. Multiple stressors are applied in vitro to enable cells to independently identify systematic adaptive solutions, thereby circumventing the pathway compensation dilemma caused by single-point intervention. Specific pathways include periodic iPSC resetting and fresh cell reinfusion to physically bypass the Hayflick limit; AI multi-omics screening to eliminate abnormal clones and control cancer risk; directed evolution of upstream progenitor cells to repair non-dividable tissues; and combined stress induction to facilitate genome-wide coordinated adaptation in cells and avoid disordered pathway compensation. Studies indicate that systematic recombination of existing technologies can deliver a low-risk, implementable engineering pathway for ultra-long healthy lifespan.