The bacterium causing urinary tract infections (UTIs), Klebsiella pneumoniae is a major globally. It is a gram-negative bacterial genus, a member of the Enterobacteriaceae family. It is a rod-shaped, non-motile, non-spore-forming with a prominent capsule made of polysaccharides. This study designed to isolate and identify Klebsiella species from urinary tract patients in Thi-Qar province. One hundred specimens were collected from UTIs-infected patients, ageing (11) to (76) years, .in three hospitals (Al-Hussein Teaching Hospital, Al-Nasiriya Teaching Hospital, and Bint Al-Huda Hospital) in Thi-Qar province between November 2023 and March 2024.The isolates were identified and their features concerning antibiotic resistance were investigated using the VITEK II system.Then examining the isolated bacterial proteins started with the polymerase chain reaction (PCR) assay identifying 16S rRNA . Results showed that rmpA, AcrAB, and fimH1 were the virulence genes. On the culture medium, the most commonly observed gram-positive bacteria were 25 (30.49%), E. coli 19 (23.17%), and K. pneumoniae 33 (40.24%).Using the VITEK II system revealed 33 samples including Klebsiella spp. bacterial presence. Men had the highest rate (77.97%) while the women rate (59.02%) regarding bacterial infections. Examining 70 drugs against K. pneumoniae, the study found that 45.67% of isolated bacteria were resistant to antibiotics, 9.91% were intermediate, and 44.42% of the bacteria were sensitive to antibiotics. Comprising 100% Ampicillin, 93.94% Amoxicillin, and 63.64% Ceftriaxone. The study revealed that successful treatments for K. pneumoniae are Cefpirome and Faropenem. The study finds isolates of 22 Klebsiella pneumoniae. PCR results of Gram-negative bacterial amplification helped to identify the isolates by means of the 16S rRNA amplification. According to the research, isolates of Klebsiella pneumoniae show great potential as targets for antibiotics and other medications. Therefore, the authors request a future study to determine the epidemiology of Klebsiella pneumoniae in all Iraqi provinces.
Salih et al. (2024) studied this question.