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September 10, 20250 citations

Carver et al, Aged Brain Spatial Profiling - Tissue Processing v1

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CCChase M. Carver

Key Points

  • This protocol details essential steps for mouse brain tissue processing.
  • It emphasizes immunohistochemistry and spatial -omic techniques for studying microglia.
  • The methods ensure accurate staining and imaging of brain sections.
  • Application of these techniques focuses on understanding changes in aged brain white matter.

Abstract

This protocol provides the steps for mouse brain tissue processing and preparation for use in immunohistochemistry and spatial -omic techniques used in Carver et al., "Senescent- and disease-associated microglia are modifiable features of aged brain white matter" The steps here should be considered as immediately proceeding the protocol for each separate process to correctly stain and image brain sections in the respective techniques.

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Cite This Study

Chase M. Carver (2023) studied this question.

synapsesocial.com/papers/68c1e25454b1d3bfb60ffc1ehttps://doi.org/10.17504/protocols.io.kqdg3x841g25/v2
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