Abstract To understand the heterogeneity of single-B cell responses to Non-alcoholic steatohepatitis (NASH), we performed Single-cell RNA sequencing (scRNA-seq) on single-B cells isolated from control and MCD-fed mice livers. Subsequent analyses included clustering, identification of differentially expressed genes (DEGs) and enrichment analysis. The expressions of high specific DEGs were validated using quantitative real-time PCR (qRT-PCR), immunofluorescence staining and function study. Four single-B cell clusters (3, 14, 16 and 20) were identified. The total number and proportion of B cells significantly decreased in NASH mice livers. In cluster 3, the decreasing Fcer2α+ mature B cells were supposed with anti-inflammatory role associated with B cell activation and differentiation of other immune cells in NASH. The DEGs (Fcer2α, Cd22, Cr2 and Fcmr) of cluster 3 were consistently downregulated in B cells cocultued with lipotoxic hepatocytes. And the portal area of livers contained fewer Fcer2α+ B cells in NASH patients and mice compared with controls. Fcer2α+ B cells attenuated lipotoxicity-driven inflammation by enhancing anti-inflammatory factor (IL-10, IL-35) secretion and inhibiting T cell inflammatory factor (IFN-γ, TNF-α, IL-17) production and proliferation. The other 3 clusters (14, 16 and 20) contained small numbers of single-B cell. Tnfrsf17+ plasmacytes (PCs) of cluster 14 were identified with the effect related to endoplasmic reticulum stress and N-Glycan biosynthesis. Klk1+ B cells of cluster 16 were implicated in regulating immune response in NASH. Apol7c+ B cells of cluster 20 participated in apoptosis, NF-κB, TNF and chemokine pathway in NASH. Thus, a subgroup of Fcer2α+ mature B cells, diminished in NASH, likely exerted anti-inflammatory or immunosuppressive effects.
Lü et al. (2025) studied this question.