This protocol describes the manual extraction of HMW DNA from animal tissue samples intended for long-read sequencing using the Nanobind® PanDNA kit and and following the ‘Extracting DNA from animal tissue using Nanobind® PanDNA kit’ procedure from PacBio. This process is effective for several animal species covered by the Tree of Life Programme, including molluscs, mammals, birds, fish and amphibians. The output of this protocol is high quality and quantity HMW DNA, which can be directed towards the Sanger Tree of Life HMW DNA Fragmentation: Diagenode Megaruptor® 3 for LI PacBio or the Sanger Tree of Life HMW DNA Fragmentation: Opentrons® OT-2 for PacBio LI protocols. Acronyms HMW: high molecular weight LI: low input
Biosciences et al. (2023) studied this question.
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