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September 6, 20240 citations

Isolation of Nuclei from Brain Tissue Using Gradient Centrifugation for Myelin Depletion for 10x Genomics Platform v1

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AIAllen Institute

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Abstract

Protocol is used for isolation of nuclei from frozen brain tissue orthawed and microdissected brain tissue sections for RNA-seq analysis. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number UM1MH130981. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health

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Allen Institute (2024) studied this question.

synapsesocial.com/papers/68e5931ab6db64358752e10fhttps://doi.org/10.17504/protocols.io.5qpvok1exl4o/v1
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