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Abstract Transmission electron microscopy (TEM) offers unparalleled atomic-resolution imaging of complex materials and heterogeneous structures. However, high-energy imaging electrons can induce structural damage, posing a challenge for electron-beam-sensitive materials. Cryogenic TEM (Cryo-TEM) has revolutionized structural biology, enabling the visualization of biomolecules in their near-native states at unprecedented detail. The low electron dose imaging and stable cryogenic environment in Cryo-TEM are now being harnessed for the investigation of electron-beam-sensitive materials and low-temperature quantum phenomena. Here, we present a systematic review of the interaction mechanisms between imaging electrons and atomic structures, illustrating the electron beam-induced damage and the mitigating role of Cryo-TEM. This review then explores the advancements in low-dose Cryo-TEM imaging for elucidating the structures of organic-based materials. Furthermore, we showcase the application of Cryo-TEM in the study of strongly correlated quantum materials, including the detection of charge order and novel topological spin textures. Finally, we discuss the future prospects of Cryo-TEM, emphasizing its transformative potential in unraveling the complexities of materials and phenomena across diverse scientific disciplines.
Wang et al. (2024) studied this question.